Unlike the existing LC-inlet solution via chemical oxidation, the iso CHROM LC works with high-temperature combustion. It quantitatively converts organic carbon to CO2.
In addition, the easy-to-use iso CHROM LC shows outstanding precision, accuracy, and linearity without significant peak broadening that can diminish the chromatographic performance. In continuous operation, virtually all peaks in a chromatogram can be analyzed for the stable isotope ratios of carbon.
The iso CHROM LC combines the proven Elementar high-temperature combustion technology with straight-forward combustion gas purification. This results in unrivaled low peak broadening that brings the chromatographic resolution of the HPLC system virtually unchanged to the mass spectrometer. This makes the iso CHROM LC the first choice of any complex separation tasks in LC-IRMS.
Thanks to the fast and complete conversion process of the high-temperature combustion, no isotope fractionation is observed. Period. This results in precise, high-quality data that can be trusted to represent the actual isotope ratios of a wide variety of samples.
The iso CHROM LC can be connected to any HPLC system to receive a sample liquid stream for conversion to CO2. Alternatively, direct injection of samples can be carried out as well to perform both bulk isotopic analysis and compound-specific analysis using the same system.