Do you have any questions?
We are here for you.
Liquid chromatograph LiquiFace
LC-IRMS compound-specific isotope analyzer
ll_to Content

High performance carbon isotope analysis of LC separated compounds

The LiquiFace is the ideal solution for research applications requiring high-performance carbon isotope analysis of dissolved organic compounds. LiquiFace offers significant benefits for versatility and ease of use in liquid chromatography isotope ratio mass spectrometry (LC-IRMS). 

Compound-specific isotope analysis of aqueous samples by liquid chromatography is highly advantageous for compound classes that are thermolabile or require derivatization making GC-based separation less attractive. Polar compounds such as carbohydrates, amino acids, alcohols, organic acids, and peptides make ideal candidates for LC-IRMS, and the technique has been employed to great success in food and biogeochemistry applications. 

LiquiFace is an interface for the LC system to provide quantitative online conversion of the carbon in the separated organic compounds to CO2, followed by extraction from the aqueous phase into the gaseous phase and subsequent δ13C analysis by the IRMS system. The instrument uses a “wet-chemical oxidation” which delivers excellent isotopic precision, accuracy, and linearity. This level of performance means that LiquiFace covers a range of sample types with a complete independence upon sample concentration, making your analysis even simpler.

Highlights of the LiquiFace

Precise δ13C analysis

achieved by optimized mixing of reagents and low-band spreading oxidation through the micro-reactor with high efficiency membrane gas exchanger

High system uptime and sample throughput

due to inherent advantages in our IRMS systems such as the highly robust, thorium dioxide-coated ionization filament and the stainless-steel analyzer housing which are substantially more robust than other instruments

Low cost of ownership

thanks to the use of inexpensive peristaltic pumps for a steady, precise flow of reaction reagents

Maximum flexibility in your LC separation

and method development thanks to the freedom to choose the LC system which suits your application best

LC-IRMS compound-specific isotope analyzer LiquiFace


Precise and accurate δ13C analysis for all compound classes

lsotopic precision, accuracy, and linearity performance is exceptional with the LiquiFace system due to the optimized mixing of reagents with the LC effluent prior to oxidation. Real-time, fractionation-free oxidation through the low temperature micro reactor ensures quantitative conversion of organic carbon to CO2 without any compromise in peak chromatography. So, no matter which compound class your research encompasses, you can be sure that the LiquiFace will deliver exceptional performance. “Bulk” δ13C isotope analysis of aqueous samples (e.g. TOC) can be performed using the in-line direct injection valve and changeable 25 μl sample loop.

High system uptime and sample throughput thanks to robust components 

Performing LC-IRMS analysis with the LiquiFace delivers superior system uptime and sample throughput compared to other wet-chemical systems. When coupled to an isoprime precisIONIRMS system from Elementar, the system has an unparalleled tolerance for excess oxygen loads, a common problem with LC-IRMS systems. With the use of sodium persulfate as oxidation agent, a large oxygen excess can enter the IRMS and damage the ionization filament of conventional IRMS systems. The unique thorium dioxide coating of the isoprime precisION ionization filament results a lifetime of years rather than months. This resistance to oxygen means that an on-line reduction furnace to remove excess oxygen is not required, reducing consumables and possible peak broadening. Also, the use of a stainless steel rather than aluminum analyzer results in significantly lower and reliable water backgrounds in the vacuum chamber, another inherent problem for LC-IRMS systems. This reduces the chance of COprotonation in the ion source which seriously compromises δ13C isotope analysis.

Low cost of ownership thanks to robust, cost-efficient components

Unlike other LC interfaces that use costly LC pumping channels to deliver reagents to the oxidation reactor, LiquiFace uses peristaltic pumps to deliver the reagents in a steady, precise flow. These on-board peristaltic pumps are easy to maintain and repair in comparison to LC pumps, offering a low cost of ownership. For maximum flexibility, the peristaltic pumps can pump up to four channels if additional reagents are required.

Maximum flexibility in your LC separation 

The LiquiFace is designed to interface with any high performance liquid chromatograph (HPLC) or ion chromatograph (IC) system, giving you maximum flexibility in choice of your LC separation system and allowing you to choose a suitable setup for method development. The connection of the LiquiFace to any standard LC or IC pumping system is realized by using finger-tight PEEKTM union connections. 

Product details

HPLC or Direct Injection Mode 

The LiquiFace can be connected to a HPLC unit to receive a sample liquid stream for conversion to CO2. Alternatively, the internal pump on the LiquiFace can be used to carry out direct injection of samples. Peristaltic pumps in the LiquiFace are used to deliver and mix reagents with the separated compounds in the LC eluent. Direct injection to the LiquiFace means that users can perform both bulk isotopic analysis and compound-specific analysis using the same system. 

Reliable peak chromatography 

The LiquiFace has been completely designed with chromatography in mind. Once your sample is separated by the LC column, then there is minimal detrimental effect of the interface on the chromatography. Therefore, for particularly complex separations the LiquiFace can be relied up on to maintain the chromatographic integrity of the separated compounds through to the isotope ratio mass spectrometer. 

Fractionation-free sample conversion 

Conversion of the aqueous eluent to sample gas is performed in a low temperature reactor at up to 100 °C. Low temperature conversion of the eluent allows our interface to analyze thermolabile samples which will disintegrate at elevated temperatures. The mixture of eluent and sample gases, generated in the LiquiFace heater, are cooled to room temperature and then separated in a gas exchanger with the remaining eluent going to a waste line and the sample gas passing through a water removal membrane in to the IRMS. 

For detection of economically motivated adulteration of honey with C3 sugars, add the Agilent 1260 Infinity LC system configured for analysis of constituent glucose, fructose, and disaccharides.

The Agilent 1260 Refractive Index Detector can be used for simultaneous quantitative analysis as well as isotopic ratio.

  • Carbohydrates

  • Amino acids

  • Biological materials

  • River waters and ground waters

  • Soil waters



Product flyer
Flyer LiquiFace
Elementar Product Portfolio Brochure
Elementar Product Portfolio Brochure
download download

These products may also interest you

Do you have any questions?
We are here for you.