Application Notes


BiovisION Honey is a complete solution for stable isotope-based honey analysis, consisting of the following modules:

Agilent 1260 Infinity II liquid chromatography system

The 1260 Infinity II LC System from Agilent Technologies is a highly reliable instrument that combines the latest column technologies and advanced supplies to guarantee robust separation and detection performance.

The LC system is specifically configured for the separation of honey carbohydrates. A pure water mobile phase is pumped by a pulse-free isocractic pump through a Phenomenex Rezex RSM-Monosaccharides Ca2+ separation column. With an autosampler capacity for up to 132 x 2 ml sample vials, your laboratory will be able to perform large unattended runs generating significantly improved return on investment.

You can find out more about the 1260 Infinity II LC from Agilent here.

isoprime visION IRMS system

ionOS® BiovisION Honey operating license for acquisition and data processing

ionOS is our advanced stable isotope software suite, designed to facilitate automatic data processing and straightforward and easy-to-use control of the entire system.

Designed completely from the ground up for stable isotope analysis, ionOS is the most sophisticated yet simple software suite ever created for this purpose. Its clean user interface belies the level of sophistication within, giving users unrivalled capabilities to generate exceptional data with ease, thanks to its automatic peak mapping and isotope difference calculations.

ionOS controls the entire BiovisION Honey system, including the Agilent 1260 Infinity II LC system, giving the operator completely integrated, intelligent control of every aspect of the analysis. With the real-time maintenance counters and detailed diagnostic tools like automated leak checking, contact user time with the instrument is significantly reduced giving more time for sample analysis.

vario ISOTOPE select elemental analysis upgrade

For users who are seeking a quick and simple EA-IRMS solution for C4 honey adulteration, the optional vario ISOTOPE select elemental analysis upgrade allows the conversion of bulk organic material to pure gases for isotope ratio analysis.

With a range of unique features for robust analysis and straightforward maintenance, the instrument is the ideal choice for compliance with the AOAC 998.12 method for the detection of C4 honey adulteration. It provides fully automated operation, with a 240-position sampler, high throughput and reduced helium consumption to minimise the cost per analysis.

The vario ISOTOPE select is also ideal for investigating the geographical origin of a honey using multi-elemental isotope analysis. 15N, 13C and 34S values can be determined quickly from a single sample analysis via its unique Temperature Programmable Desorption gas separation technique, which allows laboratories to rapidly screen samples and build an authentic sample database.

ArDB software suite

ArDB is an optional software suite that allows seamless transfer of sample information and results between the database and your instruments. Bringing together sample results for numerous analytical techniques, including IRMS, ICP-MS and TIMS, it eliminates transcription errors, duplication and inconsistent data handling.

The software facilitates the construction and maintenance of centralized sample databases offering automatic recording of calibrated analytical results for each sample, and provides a full audit trail for any changes made. It also provides an array of sophisticated analytical tools for interrogating and visualizing the data.

As such, this can be an essential tool for identifying C3 sugar adulteration of honey using LC-IRMS methods or C4 sugar adulteration via AOAC 998.12. It can also help you confirm a honey’s true geographic origin.